“Modeled Osteopathic Manipulative Treatments: A Review of Their in Vitro Effects on Fibroblast Tissue Preparations”; Manal Zein-Hammoud, PhD and Paul Standley, PhD; The Journal of the American Osteopathic Association; August 2015, vol. 115, Number 8, pp. 490-501.
Later Studies: Modeled Counterstrain and Myofascial Release Studies:
1. In these studies they combined both modeled repetitive motion strain (RMS) and modeled counterstrain (CS) and myofascial release (MFR) to investigate potential changes in human fibroblast proliferation and interleukin secretion.
“Our data revealed that human fibroblasts respond to various types of strains differently by changing cellular morphology, proliferation and cytokine and nitric oxide secretions. Although modeled RMS produced a delayed inflammatory response and reduction in cellular proliferation, both modeled CS and MFR reversed those effects.”
2. They then studied if changing the magnitude or duration of modeled MFR induced unique fibroblast responses in bioengineered tendons:
Fibroblast hyperplasia, hypertrophy, and secretion of cytokines and growth factors were examined with different MFR magnitudes (3%, 6%, 9% and 12%) and duration (0.5, 1,2,3,4, and 5 minutes):
“Bioengineered tendon weight was increased with greater-magnitude (12%) treatment, suggesting that production of extracellular matrix proteins such as collagen may be up regulated. Greater MFR magnitude also led to a statistically significant increase in IL-1B, monocyte chemoattractant cytokine, and regulated and normal T-cell expressed and secreted chemotactic cytokine compared with non-strained fibroblasts. Alternatively, holding the strain magnitude constant at 6% and varying the duration of MFR increased secretions of angiogenin, IL-3, IL-8, growth colony-stimulating factor, and thymus activation-regulated chemokine only in the 5-minute MFR group. Therefore, changing magnitude and duration of MFR appears to enhance the secretion of a unique subset of cytokines and growth factors, possibly affecting physiologic responses. Further, these findings suggest that dose-dependent and prophylactic MFR may potentially regulate inflammation and wound healing responses.”
Next week I will summarize Dr. Standley’s most recent studies that investigate effect of modeled OMT on muscle differentiation and wound healing.